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Dose increase: at Day 16 inside the on phase, the dose steps from the low end to the high end of the range

Higher concentrations (20mg/ml) minimize injection volume but make small dose adjustments less precise

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While the effects of a vitamin B12 injected will become noticeable around 24 hours after administration, they will start to take effect almost immediately and will stay in your system for around 1 week, but may vary depending on the metabolic rate of the patient

Every subsequent step of your study relies on the assumption that you're working with a known concentration