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Under a two-photon microscope, the pipette was inserted into the cortex under 200 m and the solution infused at a velocity of 0.2l/min In vivo two-photon imaging of glymphatic pathway clearance A 22mm 2 cranial window was prepared over the right parietal cortex (2 mm caudal from bregma, 1.7 mm lateral from the midline) with a micro-drill and a metal plate glued at the edge of the cranial window 16, 17

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