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however, age, racial and socioeconomic barriers were prevalent [12]

24, 25 These TJ proteins include 1 integral and transmembrane proteins, including the claudins, occludin, and junctional adhesion molecules (JAM), which extend into the intercellular spaces to regulate the gate function, and 2 cytoskeletal linker proteins, such as cingulin, ZO-1, ZO-2, and ZO-3, which anchor TJ-integral membrane proteins to cell cytoskeleton