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Freezing a mixed multi-use vial can create solution changes and freeze-thaw stress that are not worth accepting unless a protocol specifically requires aliquoted frozen storage

Then, TMB chromogen solution (CWBIO) was added, and the absorbance at 450 nm was measured via a SpectraMax plate reader (Molecular Devices)

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If you want a broader overview of the ingredient itself, What Is Liposomal Glutathione