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Conclusions The SCIEX 7500 system can quantify acylcarnitine species using LC-separation, yielding a high-throughput, robust and reproducible method that does not require derivatization of samples before analysis The SCIEX 7500 system provides unparalleled sensitivity and could be utilized to quantify other low-level acylcarnitines that are present in biological matrices without the need for extensive sample preparation or derivatization Since the SCIEX 7500 provides greater depths of sensitivity using plasma, researchers could further dilute the sample or require less sample for extraction, allowing consideration of alternative sample types for analysis