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The remaining cells were collected by trypsinization, washed three times with PBS and seeded into 6-well plates using cystine-free medium containing unlabelled GSH

Redox analysis of human plasma allows separation of pro-oxidant events of aging from decline in antioxidant defenses

GSH levels decline with age, and a lack of glutathione has been shown to leave the body more vulnerable to damage by free radicals, thus speeding up aging processes

Animal blood samples have been recovered according to EU Directive 2010/63/EU for animal experiments