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Acquisition of 1 H-NMR spectra and metabolic profiling The lyophilized metabolic extracts were suspended in 0.6 ml of deuterated water (Cambridge Isotope Laboratories Inc., Tewksbury, MA, USA) containing phosphate buffer (100 mM) and TSP (3-3-(trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid or TMSP- d 4 , Cambridge Isotope Laboratories) (0.5 mM) and inserted into a 5 mm NMR tube for immediate acquisition

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Fish Shellfish Immunol 63:334343 Lebedeva SN, Ayurzhanayeva AB, Tyheev AA, Kutyrev IA, Bazhenova BA, Zhamsaranova SD (2021) The effect of D