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Briefly, cells were lysed on ice for 15 min in NETN lysis buffer (20 mM Tris pH 8.0, 100 mM NaCl, 0.5 mM EDTA, 0.5% NP40) supplemented with 10 g/mL aprotinin, 10 g/mL leupeptin, 10 g/mL pepstatin A, 1 mM sodium orthovanadate, 1 mM sodium fluoride, and 1 mM AEBSF (4-[two aminoethyl] benzenesulfonyl fluoride)

2008 Nov;38(10):1976

However, it is important to note that these observations come exclusively from animal studies and that safety data in humans remain very limited

Will Cole, DNM, IFMCP, DC is a leading functional medicine expert who consults people around the globe, starting one of the first functional medicine telehealth centers in the world

What Is the Right Glutathione Dosage