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In each case, the microsomal suspension (1 mg protein) was incubated with 50 l smoke solution in a final volume of 20 l of 50 mM potassium phosphate buffer, pH 7.4 for 2 hours at 37 C

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The approach reflects a key challenge: e-cigarettes have only been widely used for about 20 years, meaning definitive proof based on long-term human outcomes could take decades

Smoking has a broad and detrimental impact on digestive health, ranging from microbial imbalances to heightened cancer risks