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02 Fill the machine with tobacco: Open the machine and place the tobacco evenly on the machines surface

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Differential gene expression changes in the colonic tissue transcriptome of Gpr15 -deficient mice with TNBS- and DSS-induced colitis under cigarette smoke exposure To investigate the impact of Gpr15 on gene expression in colonic tissue from mice with colitis under cigarette smoke exposure, RNA sequencing was performed on colonic tissue from both Gpr15 +/+ and Gpr15 / mice

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