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73 Increased expression or activity of ACSL4 may trigger ferroptosis in multifarious pathophysiological settings

PFIC3, progressive familial intrahepatic cholestasis type 3

Cheng B, Zhang J, Shen Q, Sun Z, Luo Y, Hu Y

One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)

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