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Fully functional N-terminal 6x-His tagged recombinant APOL1 E328C (cysteine mutant for biotin-tagging) was expressed and purified from BL21 E

Methods: Using an online survey, 3034 regular e-cigarette users (used a vaping product at least once a week for the past three months) between the ages of 16 and 24 and residing in one of ten Canadian provinces (Alberta, British Columbia, Manitoba, New Brunswick, Newfoundland and Labrador, Nova Scotia, Ontario, Prince Edward Island, Quebec, and Saskatchewan) were asked about their vaping behaviours (e.g., days vaped per week, number of vaping episodes per day, and number of puffs per vaping episode), experiences (e.g., co-use of other substances), and product preferences (e.g., nicotine concentration)

Their addiction is as real and permanent as alcoholism