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Even that little bit of neck movement was enough to let me know that perhaps I was not powerless

Pedro (Raul Arevalo) and Juan (Javier Gutierrez) surface during the feria, but parents, Rocio and Rodrigo, are not celebrating and their marriage is clearly under strain

Immunohistochemistry was performed as follows: free-floating VTA brain sections were incubated 1 h at 4 C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4 C with a mouse anti-tyrosine hydroxylase antibody (TH, Sigma, T1299) at 1:500 dilution in PBS containing 1.5% BSA and 0.2% Triton X-100 (see supplementary Table 1)

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