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Centers for Disease Control and Prevention (CDC), said there is no safe level of exposure to second hand smoke, and that people with cardiovascular disease could risk heart attack with less than an hours exposure to environmental tobacco smoke, which restricts blood vessels and increases clotting

Cigarette smoke extract (CSE) preparation CSE extract was prepared using Kentucky 1R6F research-reference cigarettes (The Tobacco Research Institute, University of Kentucky, Lexington, KY, USA)

It can be difficult to find TSP, so if you cannot find it, you may find that using TSP PF is a suitable second option

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