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Slides were then cooled to room temperature on ice and washed three times with TBST (1 TBS, 0.5% Triton X-100) before blocking in Block-B buffer (1 TBS, 3% BSA, and 0.1% Triton X-100) for 30 min at room temperature
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They were small, looked like traditional cigarettes and were primarily used by people who wanted to quit smoking, but over time developers modified the liquids to add flavors and the devices to alter the aerosol amounts
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