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RNA isolation and quantitative real-time PCR Total RNA was isolated from transgenic tobacco using an RNeasy Plant Mini Kit (Qiagen, German), following the manufacturers instructions, and cDNA was synthesized using iScript RT SuperMix (Bio-Rad, USA)

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however, soluble sugar level significantly increased in RV and free amino acid significantly increased in all root tissues (Figs

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His all-time record, he says, was a nine-day coke-assisted session of wakefulness, at the end of which he merely tipped over, slamming his head against a stereo speaker: It was just a curtain of blood. This aspect of the book, the addicts narrative, is the latest installment in a tradition that dates back to Romanticism and Thomas de Quinceys opium visions of crocodiles and other unutterable monsters, to Crabbe, Coleridge, Byron, Baudelairewhere to stop