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Our group did not find a correlation between the presence of microscopically evident nodules and the clonal character of resected parathyroid tissue, and appearances of several glands with histological patterns of diffuse hyperplasia also were unequivocally monoclonal in the absence of detectable nodular formations, suggesting that the current criteria for pathological diagnosis do not reflect the genetic differences among these two histopathological types (177)

We also graded the intensities of GPX1 staining in each insulin and glucagon cell located in 10 randomly selected islets per case by viewing, magnifying and merging image sets in Photoshop to ascertain the precise identity of the two cell types, GPX-1 positivity, grading and enumeration