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Younger adults (under 40) with good health and suspected minimal decline might start at 150-200mcg, though many will require escalation to standard doses for optimal response

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Emotional distress: Feelings of frustration, anxiety, low self-esteem, or depression often follow, as intimacy is tied to self-worth and connection

Then, the sections were coated with an anti-CD31 antibody (Santa Cruz Biotechnology, Dallas, TX) at 4 C overnight, followed by incubation with an HRP-labeled secondary antibody (Gene Tech, Shanghai, China), and the sections were visualized with a DAB system (Gene Tech)