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Briefly, hASM cells plated in eight-well chambered coverslips (Ibidi, Grafelfing, DEU) were incubated in Hanks balanced salt solution (HBSS) containing 1 M Rhod 2-AM (Thermo Fisher Scientific, Waltham, MA) for 45 min at room temperature, medium aspirated, and washed three times in HBSS and incubated further for 15 min to allow for deesterification of the Rhod 2

The tone is solicitous and rather worthy, and we are then treated to various local twee vignettes that demonstrate male supremacy and female submission

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In Cocteaus version of the story Orpheus and Eurydice are saved by Deaths self-sacrifice along with her soign assistant Heurtebise