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A total of 2 mL of saliva was collected from each participant and stored in labeled sterile cryovials with the addition of Halt Protease and Phosphatase Inhibitor cocktail (1% v/v, ThermoScientific, Waltham, MA) and stored at 80 C for downstream untargeted high-resolution HPLCMS metabolic analysis as previously described 33,34,35,36

Beitr Tabakforsch Int 10(3):149154 Ryan WS, Parrish, ME (1988)

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The remaining lifetime costs include $320,000 in health care expenses, $8,700 in increased home insurance premiums and $731,000 in lost income due to smoking issues

Smoking is also responsible for the development of fibrotic lung disease [4,5,6,7]