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Here, we used scRNA-seq to increase the understanding of the cellular dynamics and molecular profiles of resident and recruited immune cells in cigarette smoke (CS)-induced lung inflammation with special emphasis on AM and neutrophils in the alveolar space using murine smoke models that were previously shown to reflect key features of COPD including airway inflammation and pulmonary emphysema ( 2 Materials and methods 2.1 Animals Eight week old female C57BL/6J mice weighing 18-23 g (Charles River Laboratories, Sulzfeld, Germany) were used for all experiments

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2-Nitropropan

Also known as the "Twinkie diet," this approach was tried by Mark Haub, a professor of human nutrition at Kansas State University, in 2010