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This is because the cigarette is an important recurring symbol, and its significance is twofold: it represents Snake, or more specifically it stands for the role of Snake, and it also emphasizes Snakes approaching end, and his indifference towards his own life

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N-Propeptide of Type I Procollagen (PINP) and N-Propeptide of Type III Procollagen (PIIINP) were analyzed from the cell culture medium after 21 days of differentiation using Aviva systems biology (San Diego, CA, USA) PINP and PIIINP ELISA Kits according to the manufacturers instructions

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Here, Joe Camel holds a red rose for his date