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In brief, 50-100 mg leaf samples were frozen in liquid nitrogen, and ground with 1 mL Extraction Buffer [50 mM EPPS buffer, pH 8.0, containing 1 mM EDTA, 10 mM DTT, 0.1% Triton X-100 [v/v], 0.5% polyvinylpyrrolidone, and 1x SigmaFAST Protease Inhibitor Cocktail, EDTA Free (Sigma)] on ice using 2 mL glass-to-glass homogenizer

When even one hormone doesnt signal as it should, it can disrupt the delicate balance needed for reproduction

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Drugs R D 15:1320

In After 3 months, the GSE group experienced a 46% reduction in ALT, while the vitamin C group showed little change