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They tend to be ubiquitous in marine environments, having been sampled from all of the worlds oceans and as shallow as the intertidal zone to depths as deep as 9,500 meters ( Apodomenia Early attempts to amplify solenogaster nuclear ribosomal RNA genes were hindered by their unusually high GC content, which made them resistant to standard PCR ( 2 Materials and methods 2.1 Materials DNA barcode sequences were obtained for a total of 642 individual aplacophorans (566 Solenogastres and 76 Caudofoveata), broadly spanning the taxonomic diversity and global distribution of the group ( Figure 1 )